Treatment with BSZYF upregulated the expression of GnRHR in the anterior pituitary tissue. A, mRNA expression of GnRHR was analyzed by qPCR. B, Western blotting to measure the protein expression of GnRHR. The relative density was quantified relative to tubulin. Values are represented as mean±SD (n=6 per group), *P<0.05 compared with the blank group, #P<0.05 compared with the model group.